Journal: iScience
Article Title: Enhanced reverse zoonotic potential and immune evasion by omicron JN.1 variant
doi: 10.1016/j.isci.2025.112824
Figure Lengend Snippet: Transduction of various omicron S pseudovirions on 293/hACE2 and Calu3 cells (A) S proteins incorporation in pseudovirions. Pseudovirions with different omicron S proteins were pelleted down by centrifugation through 20% sucrose cushion and separated in a 10% SDS-PAGE. Detection of S proteins in pseudovirions was performed by Western blot using rabbit polyclonal anti-S2 antibodies. The p24 served as the loading controls. Experiments were done three times and one representative was shown. (B and C) Entry of pseudovirions of omicron variants. HEK 293/hACE2 and Calu3 cells were transduced with omicron S pseudovirions and lysed at 40 h post-transduction. The transduction efficiencies were determined according to luciferase activities. Experiments were done three times in triplicate and one representative is shown. The statistical difference relative to WT was determined by one-way ANOVA with Dunnett’s multiple testing correction ( n = 3). The statistical difference between BA.2.86 and JN.1 was determined by a two-tailed t-test ( n = 3). Error bars indicate SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Article Snippet: Rabbit polyclonal against SARS-CoV-2 S2 antibodies (Cat#40509-T62), rabbit polyclonal against HIV-1 Gag-p24 antibodies (Cat#11695-T62), mouse polyclonal against FLAG tag antibodies (Cat#109143-MM13) and mouse polyclonal against beta-actin antibodies (Cat#100166-MM10) for western blotting and chimeric monoclonal against SARS-CoV-2 S2 antibody (Cat#40590-D001) used for flow cytometry was purchased from SinoBiological Inc (Beijing, China).
Techniques: Transduction, Centrifugation, SDS Page, Western Blot, Luciferase, Two Tailed Test
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